2 edition of Studies on the nature and regulation of mouse hepatic glucokinase found in the catalog.
Studies on the nature and regulation of mouse hepatic glucokinase
Patricia A. James
Thesis (Ph.D.) - University of Birmingham, Dept of Biochemistry.
|Statement||by Patricia A. James.|
Reciprocal regulation of hepatic glycolysis and gluconeogenesis contributes to systemic metabolic homeostasis. Recent evidence from lower order organisms has found that reversible post-translational modification of glyceraldehydephosphate dehydrogenase (GAPDH), particularly acetylation, contributes to the reciprocal regulation of glycolysis/ by: 9. Recent studies illustrate that Hnf6-/-mouse embryos fail to develop a gallbladder and exhibit severe abnormalities in both extrahepatic and intrahepatic bile ducts (IHBD), which is associated with diminished expression of the Hnf1β transcription factor (Clotman et al., ; Jacquemin et al., ). Thus Hnf6 is essential for regulating the Author: Subhshri Sahu, Mugdha V. Joglekar, Sundy N. Y. Yang, Anandwardhan A. Hardikar.
Regulation of Glucokinase in Isolated Hepatocytes Glucokinase (Hexokinase IV or D) catalyses the conversion of glucose to Glucose 6-phosphate in liver parenchymal cells (hepatocytes) and plays an important role in the regulation of blood glucose and therefore in the disease Diabetes mellitus. Conversely, Foxo1 deletion in liver curtails excessive glucose production caused by generalized ablation of insulin receptors and prevents neonatal diabetes and hepatosteatosis in insulin receptor knockout mice. The data provide a unifying mechanism for regulation of Cited by:
Objectives Platensimycin (PTM) is a natural antibiotic produced by Streptomyces platensis that selectively inhibits bacterial and mammalian fatty acid synthase (FAS) without affecting synthesis of other lipids. Recently, we reported that oral administration of PTM in mouse models (db/db and db/+) with high de novo lipogenesis (DNL) tone inhibited DNL and enhanced glucose oxidation, which in Cited by: 6. Current Chemical Genomics and Translational Medicine (Discontinued) ISSN: ― Vol Cited by:
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The glucokinase regulatory protein (GKRP) also known as glucokinase (hexokinase 4) regulator (GCKR) is a protein produced in hepatocytes (liver cells). GKRP binds and moves glucokinase (GK), thereby controlling both activity and intracellular location of this key enzyme of glucose metabolism.
GKRP is a 68 kD protein of amino is coded for by a 19 exon gene, GCKR, on the short arm HGNC: CAHILL GF, Jr, HASTINGS AB, ASHMORE J, ZOTTU S. Studies on carbohydrate metabolism in rat liver slices. Factors in the regulation of pathways of glucose metabolism. J Biol Chem. Jan; (1)– CHERNICK SS, CHAIKOFF IL.
Insulin and hepatic utilization of glucose for lipogenesis. J Biol Chem. Oct; (2)–Cited by: In addition to regulation at the transcriptional level, GCK is also regulated in the liver at the post-transcriptional level by glucokinase regulatory protein (GCKR).
GCKR is primarily expressed in the liver , , and possibly the brain , with little if any expression in pancreatic beta-cells , .Cited by: Hepatic glucokinase activity in the C3H/He strain of mice is about twice that in the C58 strain.
Genetic analysis of hybrids and back-crosses indicates control of activity by a single codominant gene. The adaptations of activity that occur when animals of the two strains are starved, fed a carbohydrate-free diet or made streptozotocin-diabetic are similar in the two by: 6.
A recent study revealed that hepatic glucokinase overexpression in the liver of chow-fed C57BL/6 mice suppresses sympathetic nerve activity to BAT, thereby downregulating the expression of the thermogenesis-related Ucp1, Pgc1a, and Dio2 genes and modulating predisposition to sely, hepatic glucokinase knockdown in high-fat diet (HFD)-fed C57BL/6 mice attenuated Cited by: 4.
The role of insulin, glucocorticoids, and hepatic cyclic AMP in the regulation of the activity of rat liver glucokinase was investigated in following results were found: (i) Refeeding of starved rats with glucose or injection of diabetic animals with insulin resulted in a dramatic increase in the concentration of serum insulin and a decrease in the concentration of hepatic cyclic AMP Cited by: Liver glucose metabolism is dependent on glucokinase activity.
Glucokinase expression is transcriptionally regulated by hormones and metabolites of glucose, and glucokinase activity is dependent on reversible binding of glucokinase to a specific inhibitor protein, glucokinase regulatory protein (GKRP), and to other binding proteins such as 6-phosphofructokinase/fructose 2,6-bisphosphatase Cited by: As glucagon’s opponent, insulin stimulates glycolysis via enhanced expression of the hepatic glucokinase gene, 14, 15 a key enzyme that converts glucose into by: The balance of catabolic and anabolic glucose fluxes in the liver is crucial for glucose homeostasis and is disturbed in diabetes mellitus.
In this Review, the authors discuss progress in our Cited by: Hepatic GCK mRNA expression is associated with triglyceride content in human liver biopsies (r =P = ). Furthermore, hepatic GCK mRNA expression is associated with lipogenic gene expression (fatty acid synthase, r =P = ; acetyl-coenzyme A carboxylase-α, r =P =and acetyl-coenzyme A carboxylase-β, r =P = ) and the de novo lipogenesis Cited by: Abstract.
A simple method for isolation of glucokinase from a soluble fraction of rat liver was proposed, making it possible to obtain the enzyme preparation characterized by high specific activity, native cooperative characteristics of the enzyme, and actually complete absence of other molecular forms of Author: L.
Khu, N. Goncharova, A. Rubtsov. Citation: Lu M, Li P, Bandyopadhyay G, Lagakos W, DeWolf WE Jr, et al. () Characterization of a Novel Glucokinase Activator in Rat and Mouse Models. PLoS PLoS ONE 9(2): e doi The glycolytic pathway is initiated by a hexokinase that converts glucose to glucosephosphate in the cells.
Glucokinase, the rate-limiting enzyme of the glucose oxidation reaction, which acts. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. No. 13, Issue of JulyPrinted in U.S.A. Studies on the Mechanism by Which Exogenous into Liver Glycogen in the Rat A DIRECT OR AN INDIRECT PATHWAY?* Glucose Is Converted (Received for publication, Decem ) Christopher B.
Newgard, Laurence J. Hirsch, Daniel W. Foster, and J. Denis McGarryS. Glucokinase: Its Regulation and Role in Liver Metabolism (Molecular Biology Intelligence Unit) [Cardenas, Maria Luz] on *FREE* shipping on qualifying offers.
Glucokinase: Its Regulation and Role in Liver Metabolism (Molecular Biology Intelligence Unit)Cited by: In two other studies using a high‐fat feeding model in mice, a small GKA compound named GKA71 given orally for 4 weeks showed an upwards trend in hepatic TGs (Winzell et al., ), and the GKA MK‐ administered twice daily for 16 days was reported to have no effect on hepatic TGs, nor on circulating FFAs, TGs, ASAT or ALAT levels (Eiki Cited by: The liver is a critical organ of energy metabolism.
At least 10% of the liver transcriptome demonstrates rhythmic expression, implying that the circadian clock regulates large programmes of hepatic genes. Here, we review the mechanisms by which this rhythmic regulation is conferred, with a particular focus on the transcription factors whose actions combine to impart liver- and time-specificity Author: Ann Louise Hunter, David W.
Ray. Glucokinase (GCK) 1 plays a critical role in the regulation of insulin secretion and has been termed the pancreatic β-cell glucose sensor on account of its kinetics, which allow the β-cells to change glucose phosphorylation rate over a range of physiological glucose concentrations.
These kinetic characteristics are the enzyme's low affinity for glucose (S ∼ m m), cooperativity with. Insulin-like growth factor II (IGF-II), a polypeptide hormone with structural homologies to insulin-like growth factor I (IGF-I) and insulin, regulates the metabolism and growth of many tissues.
In this study, we examined the role of IGF-II in hepatic glycogen metabolism in normal Cited by: Read "Hormonal Regulation of Hepatic Gluconeogenesis and Glycolysis, Annual Review of Biochemistry" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Hepatic GCK tightly controls hepatic glucose disposal and is a major contributor to glucose homeostasis. GCK gene expression and activity are regulated by translational and post‐translational mechanisms.
Insulin is the primary up‐regulator of the GCK gene promoter, probably acting through the PI3K/Akt by: Recent studies have also suggested that hepatic insulin signaling sustains LDLR levels.
We therefore sought to elucidate the mechanisms linking hepatic insulin signaling to regulation of LDLR levels. In WT mice, insulin receptor knockdown by shRNA resulted in Cited by: Thus, hepatic insulin signaling is essential to the maintenance of energy homeostasis through the regulation of glucose and lipid metabolism.
In the insulin-resistant state, when insulin no longer regulates carbohydrate and lipid metabolism, hyperglycemia, hepatic steatosis and dyslipidemia by: